If the motherboard is the same type as the old one, you should be fine.
If you are upgrading to a newer motherboard (different socket) with a new processor 
and/or new RAM, then you will have to reinstall XP.
If the "C:" drive is a physically separate drive from the "E,F,and G" drive(s) then 
disconnect the "E,F,and G" drive(s) and leave only the "C:" drive connected to the 
motherboard.
You may have to change the jumper back to default on the "C:" drive.
If you have a single hard drive partitioned "C,E,F,and G" then follow TekNurds advice.

Good luck!

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